Analysis of Piper sarmentosum proteome using two dimensional gel electrophoresis and mass spectrometry
نویسندگان
چکیده
* Author for correspondence: Professor Lay-Harn Gam, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia. Tel.: 604-6532208, Fax: 604-6570017, Email [email protected]. Abstract. P. sarmentosum is a traditionally used medicinal plant. Protein profiling of leaf extracts of P. sarmentosum was performed using two-dimensional electrophoresis (2-DE). The 2-DE patterns showed that most of the protein spots were detected in the mass range of 10-90 kDa on a pH 4-7 IPG strip. A total of 239 protein spots were detected, the spots were excised and digested with trypsin and subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS) for protein identification, from which, 276 proteins were identified. 6.2% of these proteins were categorized as defense and stress-related proteins. Many proteins in the category had been reported to possess antioxidant and anti-pathogen properties.
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